Yields of expression and purification of the recombinant semi-proPbPM4 from one liter culture.

aThe product yield of each step was the result from three individual expressions, presented as mean ± SEM.bWeights were directly measured using a balance after centrifugation.cWeights were directly measured using a balance after purification.dThe concentration of soluble protein was determined using OD280 (ε280 = 41,510 M-1 cm-1, a theoretical value calculated from the sequence of semi-proPbPM4 using ProtParam [58]).Yields of expression and purification of the recombinant semi-proPbPM4 from one liter culture.