Identification of Genomic Safe Harbors in the Anhydrobiotic Cell Line, Pv11

Genomic safe harbors (GSHs) are utilized as an ideal integration site for generating transgenic organisms and cells. Discovery of GSHs is one of the crucial factors for the advancement of basic and applied biology in the species. Such GSHs were discovered in Pv11 (Polypedilum vanderplanki) cell line, which can survive extreme desiccation. To identify the integration sites, high-molecular-weight genomic DNAs were extracted. The DNA libraries were prepared and sequenced with a Nanopore MinION sequencer. In the way to confirm that GSHs loci are localized in open chromatin regions we prepared ATAC-seq libraries, which were sequenced in Illumina HiSeq2500. Overall design: Two libraries with Nanopore sequencing for GSHs and six libraries with ATACseq Illumina PE reads: 3 controls (K) and 3 trehalose treated Pv11 cells (T).