Effects of FGFRL1 knockdown on gene expression in ovarian cancer cell lines

Analysis of ovarian cancer cell lines after knockdown of FGFRL1 using SiRNA. To elucidate the signaling pathways that were significantly altered following the silencing of FGFRL1 expression, we performed global gene profiling experiments of the OVCAR8 and ES2 cells after knockdown of FGFRL1 using siRNA. We conducted pathway analysis with the differentially expressed genes using R in two OC cells. Ovarian cancer cell line OVCAR8 and ES2 were transiently transfected with control or FGFRL1 siRNA, followed by RNA extraction by RNA extraction and analyses wth the Affymetric Human U133 Plus 2.0.