Human Lung Fibroblasts treated with TGFbeta, IL1, EGF and small molecule inhibitors of TGFBR1 and p38. Homo sapiens

Experiment conducted to reverse engineer the transriptional response network of cytokines, growth factors and small molecular inhibitors in HLF cells. Global gene expression was analysed by microarrays Overall design: HLF cells were incubated with each agent for 0.5 hours, 1 hour, 2 hours or 8 hours in standard conditions before total RNA was prepared and hybridised to Affymetrix microarrays