Data_Sheet_1_Practical Method to Screen Contaminated Holograms of Flocs Using Light Intensity.pdf

Submergible digital holographic camera can measure the in situ size and shape of suspended particles, such as complex flocs and biological organisms, without disturbance. As the number of particles in the water column increases, overlapping concentric rings (interference patterns) can contaminate the holographic images. Using light intensity (LI), this study proposes a practical method to assess the degree of contamination and screen out contaminated images. The outcomes from image processing support that LI normalized on a gray scale of 0 (black) to 255 (white) can be a reliable criterion for defining the contamination boundary. Results found that as LI increased, the shape of the particle size distribution shifted from a positively skewed to a normal distribution. When LI was lower than approximately 80, owing to the distortion of particle properties, the settling velocities derived from the contaminated holograms with mosaic patterns were underestimated compared to those from the uncontaminated holograms. The proposed method can contribute to a more accurate estimation of the transport and behavior of cohesive sediments in shallow estuarine environments.