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Identification and quantification of JIP4 phosphorylation sites upon acrolein stimulation.

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JIP4-mycDDK overexpressing SH-SY5Y cell treated with acrolein with or without Jak3 inhibitor VI. JIP4-mycDDK was partially purified with anti-flag M2 magnetic beads. After reduction and alkylation of the samples, trypsin/Lys-C digestion were performed. Identification and quantification of the phosphorylation site of JIP4 by acrolein were analyzed using a mass spectrometer.
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2022-10-01
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