Cytotoxicity and mechanism of Programmed cell death of Coleus amboinicus (Lour) against Colon Cancer Cell

Coleus amboinicus (Lour) (CA) has been reported to possess many pharmacological activities. In this study, evaluation of cytotoxicity using brine shrimp lethality bioassay and MTT assay using WiDr cell lines was carried out. The expression of several genes responsible for programmed cell death of the methanol extract of CA was also investigated. The morphology of the cells that was undergoing apoptosis was detected using Hoechst staining assay. The gene expression of Bax, Bcl-2, p53, caspase 1, 7, 8 and 9 of treated samples with different concentrations (10, 15, 25 & 50 µg/ml) were measured with RT PCR.