Discovery of a periosteal stem cell mediating intramembranous bone formation and fracture healing (batch RNA-Seq)

FACS sorted 4 bulk cell population from mouse femur (P6) were subjected to RNA sequencing. The samples were periosteal stem cell (PSC), progenitor population type 1 (PP1), progenitor population 2 (PP2) and CTSK-mGFP negative mesenchymal stem cells (MSC). The aim of this project was to obtain Differentially expressed gene list for each cell types as well as compare transcriptinal profile between each population. All samples for RNA seq were obtained on the same day from mouse femur within the same litter. Cells were sorted into 4 populations by FACS. No samples were pooled. There are 4 Replicates for each populaton.