Alba-domain proteins translocate from the cytoplasm to the flagellum and the nucleolus upon Leishmania amastigote differentiation.

Subcellular localization of LiAlba1-HA and HA-LiAlba3 proteins in promastigotes (A) and upon amastigote differentiation (8 h in MAA medium pH 5.8 at 37°C) (B) was assessed by indirect immunofluorescence studies using an anti-HA antibody as described in Materials and Methods. DAPI staining (red) allows detection of the nucleus (N) and kinetoplastid DNA (K). C) Immunofluorescence images of wild type L. infantum episomally co-expressing pSP-NEOalphaIR-eYPF-LiAlba1 and pSP-HYGalphaIR-mCh-LiNOP10 grown as promastigotes (Pro), differentiating amastigotes (Diff) and amastigotes (Ama). LiNOP10 was used as a nucleolar (Nu) control.