MiRNA expression in embryonic murine hepatocytes versus murine cholangiocytes at E18.5

Differentially expressed miRNAs between hepatocytes (GFP-neg in SOX9-GFP mice) and cholangiocytes (GFP-pos in SOX9-GFP mice) in mice at E18.5. We want to compare miRNA population in biliary cells and parenchymal cells (depleted from hematopoietic cells) during liver development. Cholangiocytes specifically express the transcription factor Sox9. This enables to separate cholangiocytes from the rest of the liver parenchyma by FACS from Sox9-GFP transgenic mice. In conclusion, we want to compare miRNA population in our GFP+ cells (cholangiocytes) and our GFP- cells (liver parenchyma depleted from hematopoietic cells). 200 ng of RNA were labelled and hybridized to miRCURY LNA array (7th Gen) by Exiqon S/A Profiling Services. Three biological samples, each representing a pool of 20 livers, of Sox9-GFP+ and Sox9-GFP- cells labelled with Hy3 or Hy5 were compared. miRNAs with Benjamini-Hochberg adjusted p-values < 0.05 were selected.