MNase sensitivity of promoter chromatin in GM12878 cells during stimulation with heat-killed Salmonella typhimurium

We employed an MNase-Transcription Start Site Sequence Capture method to map and determine the accessibility of all nucleosomes during immune stimulus, at high coverage for all human Pol II promoters. We uncovered features of nucleosomal organization and sensitivity to MNase digestion in B-lymphoblastoid cells. We also find that transcription factor binding is associated with sensitive nucleosomes. Overall design: Nucleosome mapping via MNase-seq during heat-killed Salmonella typhimurium (HKST) stimulation in GM12878 cells.