Effect of depletion of LEMD3 on histone modification H3K27ac of A7r5 vascular smooth muscle cells [ChIP-seq]

The contractile phenotype of vascular smooth muscle cells is essential for maintaining vascular homeostasis.In this study, we found that LEMD3, a nuclear membrane protein, may be involved in maintaining the contractile phenotype of vascular smooth muscle cells through a genome-scale CRISPR screen. To further explore the impact of Lemd3 knockdown on global landscape of H3K27ac and H3K9me3, we performed ChIP-sequencing of scrambled siRNA-transfected and Lemd3 siRNA-transfected rat VSMCs. A7r5 cells were transfected with scrambled siRNA or siRNA targeting Lemd3.