microRNA-seq reveals microRNAs under the regulation of miR-125b in NCCIT tumor cells

To investigate the role and mechanism of miR-125b on NCCIT cells without bias, we analyzed differentially expression microRNA profile among miR-125b antagomir-, miR-125b agomir-, and negative control-transfected NCCIT tumor cells by microRNA-seq. Total RNA (1 μg for each samples) was extracted from miR-125b antagomir-, miR-125b agomir-, and control oligonucleotide (GenePharma, Shanghai, China)-transfected NCCIT (ATCC® CRL-2073) cells using miRcute miRNA Isolation Kit (Tiangen, Beijing, China). Three duplicate samples for each treatment were included. Then, equal amounts of total RNA were analysed using BGISEQ-500 platform and the sequencing procedure was performed by BGI (Shenzhen, China)