Dataset concerning the study of RBMXL3 protein

1. Confocal microscopy of wild-type TCam-2 cells and TCam-2 cells expressing RBMXL3 performed using anti-FLAG and anti-DDX39B (HPA) antibodies. 2. Confocal microscopy of wild-type TCam-2 cells and TCam-2 cells expressing RBMXL3 performed using anti-FLAG and anti-DDX39B (Proteintech) antibodies. 3. Confocal microscopy of wild-type TCam-2 cells and TCam-2 cells expressing RBMXL3 performed using anti-FLAG and anti-EIF4A3 antibodies. 4. Confocal microscopy of wild-type TCam-2 cells and TCam-2 cells expressing RBMXL3 performed using anti-FLAG and anti-RBMXL3 antibodies. 5. Immunohistochemistry of RBMXL3 protein in the normal adult human testis. 6. RT-qPCR data for relative quantification of RBMXL3 and GFP expression in reference to ACTB and GAPDH in wild-type TCam-2 cells and TCam-2 cells stably expressing RBMXL3 or GFP protein. 7. Western Blot of TCam-2 cells detecting RBMXL3 protein (band around ~140 kDa) with the use of anti-FLAG, anti-RBMXL3, and anti-RBMXL2 antibodies. GAPDH served as a loading control. Order of samples on the membrane: TCam-2 cells expressing RBMXL3, wild-type TCam-2 cells. 8. Western Blot of TCam-2 cells expressing RBMXL3 detecting RBMXL3 protein (band around ~140 kDa) with the use of serum-containing and serum-free specific RBMXL3 antibody. GAPDH served as a loading control. 9. Western Blot of TCam-2 cells after immunoprecipitation using magnetic beads coated with anti-FLAG antibody detecting RBMXL3 protein (band around ~140 kDa). Order of samples on the membrane: TCam-2 cells expressing RBMXL3_FLAG (input, flow-through, and immunoprecipitated fraction), GFP_FLAG (input, flow-through, and immunoprecipitated fraction) and wild-type TCam-2 cells (input, flow-through, and immunoprecipitated fraction). 10. Western Blot of TCam-2 cells detecting RBMXL3 protein (band around ~140 kDa) with the use of anti-FLAG antibody. GAPDH served as a loading control. Order of samples on the membrane: TCam-2 cells expressing RBMXL3_FLAG, GFP_FLAG, and wild-type TCam-2 cells.