Blood transcripts from female Japanese quail given LPS injection or saline

The aim of this experiment was to test how female Japanese quail respond to being housed with sick (LPS treated) relative to control males. LPS injected females were also tested. Adult female Japanese quail received either saline or LPS injection. All animals were allowed to interact with males for 3 h. Half of the saline injected birds were exposed to LPS injected males and the other half to control males. A blood sample was collected from the brachial vein at the end of the 3h. Blood was spun down and plasma and red blood were separated. RNA was extracted from red blood portion.