miRNA sequencing of extracellular vesicles derived from podocytes under high glucose stimulation.. miRNA sequencing of extracellular vesicles derived from podocytes under high glucose stimulation.

Extracellular vesicles were isolated from the cell culture supernatants of podocytes under high glucose(HG), normal glucose(NG) and iso-osmolality stimulation (three replicates/group). miRNA sequencing was performed to identify differentially expressed miRNAs in podocyte-derived extracellular vesicles. After sequencing, A total of 1915 miRNAs were annotated from all samples . A comparison of the HG and NG groups showed that 11 miRNAs were differentially expressed (4 for up-regulated and 7 for down-regulated,|log2(fold change)| > 1, p-value 1, p-value < 0.05). This study provides the results of miRNA alteration in podocytes extracellular vesicles under HG stimulation. Overall design: Examination of miRNA expression profiles of podocyte-derived extracellular vesicles using high-throughput sequencing.