Clara Franzini-Armstrong, Kathryn Loesser (2011) CIL:36130, damselfly, flight muscle cell. CIL. Dataset

Longitudinal and cross sections showing ribbon like myofibrils regularly alternating with layers of mitochondria and SR/T tubules. Dyads are located in a grove of the mitochondrial surface. Negatives aere scanned on nikon 8000 at 4000dpi/6.3 micron scan step size, then reduced in size 4X for upload, for an effective pixel size of 25 micron.

Technical details:
Heads and tail removed, thorax bisected and immersed in 3% glutaraldehyde buffered in 0.1 M cacodylate buffer (pH7.2). Stored at 4C for several days. Muscle dissected out. Rinsed 3Xin buffer, postfixed in 2% PsO4 in buffer, for 1.5 hrs; rinsed 3 times in water, en-block stained in saturated aqueous uranyl acetate, embedded in Spurr. Thin sections stained in uranyl acetate and lead and examined in a Philips 410 Microscope.

Reference for the image:
Loesser, K.E., Castellani, L. and Franzini-Armstrong, C. Disposition of junctional feet in muscles of invertebrates. J. Muscle Research Cell Motility 13:161-173, 199

纵向及横断面图像展示了如带状肌原纤维与线粒体层和SR/T细管的有规律交替排列。二联体位于线粒体表面的凹槽中。负片在尼康8000相机上以4000dpi/6.3微米扫描步长进行扫描，然后缩小4倍以备上传，有效像素尺寸为25微米。

技术细节：
头部和尾部被移除，胸部被横切并浸泡于0.1M水杨酸缓冲液（pH7.2）中的3%戊二醛中。在4°C条件下储存数日。肌肉组织被分离出来，用缓冲液冲洗3次，然后使用2%磷酸盐溶液在缓冲液中固定1.5小时；用水冲洗3次，整体在饱和的醋酸铀水溶液中染色，并采用Spurr方法进行包埋。薄切片在醋酸铀和铅染剂中染色，并在Philips 410显微镜下进行观察。

图像参考文献：Loesser, K.E., Castellani, L. 和 Franzini-Armstrong, C. 无脊椎动物肌肉中连接脚的分布。J. Muscle Research Cell Motility 13:161-173, 1992。