Discovery and validation summary for identified βTRCP substrates.

* indicates that the protein appears stable even in the absence of the cullin inhibitor MLN4924. The closest to consensus βTRCP degron found in the primary sequence of each novel candidate is shown.
List of all proteins purified uniquely and at least twice by the βTRCP Ligase Trap, with total spectral counts (TSC) for each of three purifications. Two unique HLA alleles were excluded, as other HLA alleles were identified in negative control purifications. Substrates in normal text were previously well-described, and those in bold are novel, although some have previously been isolated in large-scale experiments. The substrates below the black bar are known βTRCP substrates that were isolated, but did not meet our criteria for candidates. For the validation experiments, a blank box means the experiment was not performed.