Single pollen nuclei sequencing data of Hordeum bulbosum clone FB19-011-3

Anthers with mature pollen grains of 20 – 30 H. bulbosum FB19-011-3 florets were collected in and the corresponding nuclei were isolated by applying the filter bursting method. Illumina NGS libraries were prepared from 96 single pollen nuclei using the PicoPLEX Gold Single Cell DNA-Seq kit (R300670, Takara Bio USA, Inc.). A narrow size range of the pooled library (approximately 300 – 500 bp) appropriate for sequencing using a patterned flowcell was excised and purified. After the addition of 1,5 % PhiX DNA as a control, the final pooled library was sequenced (SP flowcell, 2x 151 cycles paired-end, dual-indexing with 8 cycles per index) using the Illumina NovaSeq 6000 instrument.