Supporting datasets for studies.

Duplicated Cq values from Cx. pipiens mosquitoes intrathoracically (Brookwood line) inoculated with Schmallenberg virus and then processed immediately using sqPCR (Table A in S1 File). Duplicated Cq values from Cx. pipiens mosquitoes (Brookwood line) intrathoracically inoculated with Schmallenberg virus and then processed following a 14 day incubation period using sqPCR (Table B in S1 File). Duplicated Cq values from Cx. pipiens mosquitoes (Brookwood line) intrathoracically inoculated with Schmallenberg virus and then processed following a 14 day incubation period. Saliva from each mosquito was collected using a glass capillary tube and insecticidal treatment and the body was then dissected into head and abdomen/thorax before processing using sqPCR (Table C in S1 File). Cq values from Cx. pipiens mosquitoes (Brookwood line) fed through a membrane on a Schmallenberg virus/blood suspension and then processed immediately using sqPCR (Table D in S1 File). Cq values from Cx. pipiens mosquitoes (Brookwood line) fed through a membrane on a Schmallenberg virus/blood suspension and then processed after a 14 day incubation period using sqPCR. A total of 92 samples returned no Cq value or a value >40 (Table E in S1 File). Cq values from Cx. pipiens mosquitoes (Caldbeck line) fed through a membrane on a Schmallenberg virus/blood suspension and then processed immediately using sqPCR (Table F in S1 File). Cq values from Cx. pipiens mosquitoes (Caldbeck line) fed through a membrane on a Schmallenberg virus/blood suspension and then processed after a 14 day incubation period using sqPCR. A total of 71 samples returned no Cq value or a value >40 (Table G in S1 File). Cq values from Cx. pipiens mosquitoes (Caldbeck line) fed through a membrane on a Schmallenberg virus/blood suspension and then processed following a 14 day incubation period. Saliva from each mosquito was collected using a glass capillary tube and insecticidal treatment and the body was then dissected into head and abdomen/thorax before processing using sqPCR (Table H in S1 File). Cq values from Cx. pipiens mosquitoes (Wageningen line) fed through a membrane on a Schmallenberg virus/blood suspension and then processed after a 14 day incubation period using sqPCR. A total of 113 samples returned no Cq value or a value >40 (Table I in S1 File).
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