Transcriptome of Actinosynnema pretiosum ATCC 31280 under exogenous supplement of AP-3

In search of AP-3 resistant genes, ATCC 31280△ansaA (inactivation of ansamitocin pksA, in avoiding of the interruption of endogenous generated AP-3) were cultured under the addition of 0, 50, 100 and 200 mg/L exogenous AP-3 for 24 h and collected, for strains under 200 mg/L AP-3, samples after 48 h and 72 h were also collected, and the RNA samples were collected and sent for sequencing. Exploration of the expression profile of genes under exogenous addition of AP-3 and search for ansamitocin resistant genes.