Altered lncRNA involoved in the development of lupuis nephritis

As a newly discovered type of long non-coding RNAs (lncRNAs), we aimed to assess the expression profiles of lncRNAs in children with lupus nephritis (LN) by microarray analysis. 6 venous blood samples were obtained from LN patients and controls for this study. Sequencing of small RNAs was performed to evaluate the expression profiles of lncRNAs and mRNAs in these two groups. We identified 502 altered lncRNAs and 291 mRNAs in LN patients compared to healthy controls. A coding-non-coding gene co-expression (CNC) network profile based on eight validated altered lncRNAs as well as 258 interacted mRNAs. Following by Gene Ontology analysis, the target genes of the lncRNAs were most enriched in neutrophil degranulation, secretory granule, and catalytic activity. In addition, Kyoto Encyclopedia of Genes and Genomes pathway analysis reported that the target genes were most enriched in NOD-like receptor signaling pathway and Th1/Th2/Th17 significant pathway. The present study showed that lncRNAs were significantly altered in children with LN compared to healthy controls and may play a critical role in the development and pathogenesis of LN. To assess the expression profiles of lncRNAs and mRNAs in children with lupus nephritis (LN), we use PBMC from LN children