Transcriptional changes to cytotoxic stresses are driven by HSF-activated genes and enhancers [ChIP-seq]

We utilized chromatin immunoprecipitation sequencing (ChIP-seq) to analyze the binding of HSF1 and HSF2 to chromatin under oxidative stress and heat shock. ChIP-seq was performed in mouse embryonic fibroblasts (MEFs) that were exposed to heat shock (HS) or oxidative stress induced by menadione (MD). Antibodies against HSF1 and HSF2 were used for immunoprecipitation. 2 replicates of ChIP-seq were performed in WT MEFs that were treated with HS (42 °C, 1 h) or MD (30 µM, 2 h). Antibodies against HSF1, HSF2 were used for ChIP-seq. IgG and Input are provided for the normalization of the data. Please note that each processed data file was generated from both replicates and is linked to the corresponding Rep1 sample records.