ChIP-seq data of PA3299 in Pseudomonas aeruginosa PAO1

Quorum sensing (QS) is a ubiquitous cell-to-cell communication mechanism in bacteria. Here, we demonstrate that the long-chain fatty acid-CoA ligase FadD1 could sense the DSF-type QS signal cis-DA and functions at the top of the QS hierarchy of Pseudomonas aeruginosa, as it controls the transcriptional expression of lasR and the production of AHLs and PQS signals. The significance is to identify FadD1 as the signal receptor of cis-DA with a DNA-binding leucine zipper motif to control the expression of various target genes. Our study reveals a new list of response regulators of QS signal composed of a specific class of fatty acid-CoA ligases with a leucine zipper motif that play a vital role in the physiology and pathogenesis of bacteria.