Fig. 2 Images for JAnaP/IJOQ Analysis

Tight junction disruption is a hallmark of blood–brain barrier dysfunction. Thus, the quantification of tight junction integrity is important for understanding the mechanisms of BBB and CNS pathologies. Our comparison of two automated cell–cell junction analysis programs highlights the utility of both programs as a means of studying tight junction disruption in brain endothelial cells. Additionally, this study demonstrates the advantages of each program and suggests contexts in which one or the other may be more appropriate or beneficial. By evaluating the tools available for tight junction analysis, this study will promote further investigation of blood–brain barrier disruption and CNS disease.