Supplementary table 4 - Statistical analysis on transcriptomic data

In order to evaluate the individual effects of the seed developmental stage (Globular, Transition, Torpedo, Bent cotyledon and Mature green), and development condition (Heat stress : 27°C/24°C and Control : 21°C/19°C) on gene expression, a multifactorial analysis was conducted using the multifactor designs method of the DEseq2 R package (Love et al., 2014)( https://bioconductor.org/packages/release/bioc/html/DESeq2.html). This method evaluates the weight of each factor considered in the analysis and its impact on differentially expressed genes (DEGs), according to a false discovery rate (FDR)-adjusted p-value <0.05.