Spatial total RNA sequencing of formalin-fixed paraffin embedded tissue by Spatial Random-seq

Current spatial transcriptomic methods have been widely used to resolve gene expression; however, these methods are limited to fresh or fresh-frozen samples due to unsuccess of oligo(dT) primers in degraded RNA samples. Here we develop a spatial random-sequencing (spRandom-seq) technology for Formalin-fixed paraffin-embedded (FFPE) tissues by capturing full-length total RNAs with random primers. This approach provides a powerful spatial platform for clinical FFPE specimens and promises enormous applications in biomedicine. Spatial single cell RNA-sequencing of FFPE mouse tissues using spRandom-seq , benchmark analysis was performed.当前空间转录组学方法已被广泛应用于基因表达解析，但由于寡聚(dT)引物在降解RNA样本中无法有效发挥作用，这类方法仅局限于新鲜或新鲜冷冻样本。本研究针对福尔马林固定石蜡包埋（Formalin-fixed paraffin-embedded, FFPE）组织，开发了空间随机测序（spatial random-sequencing, spRandom-seq）技术，该技术通过随机引物捕获全长总RNA。此方法为临床FFPE样本提供了强有力的空间组学研究平台，在生物医学领域具备巨大的应用潜力。本研究利用spRandom-seq技术对FFPE小鼠组织开展空间单细胞RNA测序，并完成了基准分析。