Gene expression in COPD and non-diseased nasopharyngeal organoids following Pseudomonas aeruginosa infection

Pseudomonas aeruginosa is a common bacteria leading to exacerbations of chronic obstructive pulmonary disease (COPD) patients while this bacteria can be easily eradicated by the immune systems of healthy individuals. Human airway organoids derived from healthy individuals and COPD patients were infected with pseudomonas aeruginosa. This project aims (1) to understand the differences in gene expressions in healthy and COPD airway organoids during stable condition, without infection and (2) to investigate differential pathogenic mechanism (i.e. antimicrobial defense) of pseudomonoas aeruginosa infection in healthy and COPD populations. Three healthy donors and three COPD patients were included in this study and samples were collected with and without pseudomonas aeruginosa infection. Overall design: Comparative gene expression profiling analysis of RNA-seq data for COPD and non-diseased nasopharyngeal organoids, with or without infection of Pseudomonas aeruginosa