Oocyte transcriptome changes in Taf4b-deficient female mice [E16.5]

Research has shown that Taf4b-deficient female mice display excessive perinatal germ cell death, delayed germ cell cyst breakdown, and increased chromosome asynapsis. Therefore, we hypothesized that TAF4b, as part of TFIID, regulates oogenesis and meiotic gene programs. However, the transcriptomic effects of Taf4b-deficiency and how this may lead to the infertility we observe in mice has not yet been studied. Therefore, we performed RNA-seq to examine gene expression changes in E16.5 female Taf4b-heterozygous and Taf4b-deficient germ cells E16.5 oocytes were obtained through fluorescence-activated cell sorting (FACS) from Taf4b-heterozygous and Taf4b-deficient germ cells with 5 replicates per genotype