qPCR results for gene expression in pigs with different shedding phenotypes at day 0 versus day 2 following ST inoculation, comparing with microarray data.

aFC, fold change: the ratio of the Ct value (qPCR) or probe intensity (microarray) between contrasts. FC greater than 1.0 indicates the gene was up-regulated, FC less than 1.0 indicates the gene was down-regulated for the two samples shown.
*Statistical test of difference between contrasts: *indicates trends to significance at p<0.1 (Student's t-test), **indicates significant difference at p<0.05.
#All genes shown were selected for qPCR validation. The genes were selected with respect to their rank of expression changes (#q<0.1, fold change>1.5 or <0.66; ##q<0.05, fold change>1.5 or <0.66;), as well as biological function, quality of annotation, available nucleotide sequence length, and estimated microarray signal intensity as an indicator of transcript abundance.