Additional file 2 of Expanding the CRISPR/Cas genome-editing scope in Xenopus tropicalis

Additional file 2: Table S1. Computationally identified total number of potential off-target sites in Xenopus tropicalis genome with up to 5 mismatches to 7 targets. Table S2. 23 potential off-target sites selected for T7EI assay and the corresponding PCR primers for T7EI assay. Table S3. Sequences of all Cas9s tested in this study. Table S4. List of gRNA targeting sequences for various SpCas9 variants, PCR primers for the amplification of gRNA transcription templates, and PCR primers for detecting the targeted loci. Table S5. List of SaCas9 and KKH SaCas9 gRNA targeting sequences, PCR primers for the amplification of gRNA transcription templates, and PCR primers for detecting the targeted loci. Table S6. List of LbCas12a crRNA targeting sequences, T7 primer and single-stranded oligodeoxynucleotides used for the generation of crRNA transcription templates, and PCR primers for detecting the targeted loci.