Identification of PARD3 signature on PARD3 deficient H157 cell line, reconstituting the expression of PARD3 gene, with a wt and a mutant form.. Homo sapiens

To development our gene expression approach, we have employed whole genome microarray expression profiling as a discovery platform to identify genes potentialy regulated by the polarity protein PAR3. Human SCC cell line, NCI-H157 (H157), has a big deletion at PARD3 locus and showed no PAR3 protein expression. This cell line was used as a model in which we restored the expression of PAR3 (wt or mutant form). Afterwards, this model was used to identify PARD3 signature on Human SCC. Overall design: There are triplicates of each sample. We compare the effects in gene expression between the different situations: no PARD3 expression in the cell, expression of the mutant form of PARD3 gene and expression of the PARD3 wt form in the cells.