Quantification of overlap between the direct CtrA, ChpT, CckA, DivK, PleC and DivJ regulons enables a reconstruction of the signal flow through the CtrA pathway.

Shown is an overview of the genes from the direct CtrA regulon that are present in the ChpT, CckA, DivK PleC and DivJ regulons. The thresholds for inclusion in a regulon were a log2-fold change of 1.3 for CckA and ChpT and log2-fold change of 0.5 for DivJ, PleC and DivK. Based on the overlap between the different regulons, the signal input and output at the different nodes and the signal flux between the different nodes in the regulatory cascade were quantified as a percentage of the total signal input into CtrA. (XLSX)