Vitamin D receptor and STAT3 cooperate to TET2-mediated acquisition of tolerogenesis [DNA methylation profile]

The active form of vitamin D, 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), induces stable tolerogenesis in dendritic cells (DC). This process involves the Vitamin D receptor (VDR) which translocates to the nucleus, binds its cognate genomic sites and promotes epigenetic and transcriptional remodeling. In this study, we investigated the interplay between the vitamin D receptor (VDR) and transcription factors to induce DNA methylation changes, which might provide phenotypic stability to the tolerogenic phenotype of DCs. DNA methylation profile with EPIC arrays (Illumina) on human monocytes (MO) and monocyte-derived dendritic cells in the absence (DC) or presence (tolDC) of the active form of vitamin D (calcitriol)