RNA-seq for inflamed and non-inflamed mucosa in patients with Crohn's disease

To investigate the gene expression profile of inflamed and non-inflamed mucosa in patients with Crohn's disease. To investigate TCR repertoires in the intestinal mucosa, the expression profile of the TCR repertoire gene was analyzed. We prospectively enrolled 13 patients with active CD (mean age: 30.9 ± 10.9 years; 9 were males) who underwent diagnostic or surveillance colonoscopy. Clinical characteristics of the enrolled patients were as follows: Montreal classification A1 / A2 / A3, n = 2 / n = 8 / n = 3; L1 / L2 / L3, n = 6 / n = 2 / n = 5; B1 / B2 / B3, n = 5 / n = 5 / n = 3. During colonoscopy, 2-4 biopsy samples were taken from active ulcer margins (inflamed intestinal mucosa, n = 13) and endoscopically normal-appearing mucosa (non-inflamed intestinal mucosa, n = 13) from each patient with CD. As controls, 9 non-IBD patients were enrolled prospectively (mean age: 49.7 ± 14.9 years; 6 were males) who underwent colonoscopies for colorectal polyp removal. Endoscopically normal-appearing healthy colonic mucosa specimens were obtained from the ascending colon of each non-IBD control (healthy mucosa of non-IBD control, n = 9). *** Submitter declares that institutional policy restricts the deposit of patient raw data outside the institution. ***