Anti-TGFβ antibody inhibits doxorubicin-mediated increase in osteoclast formation.

Mononuclear cells from spleen (A) and bone marrow (B) from C57BL/6 were isolated and cultured for 15 days in the presence of MCSF and RANKL until mature osteoclasts are formed and scored using TRAP staining as described in Materials and Methods. (C) Osteoblast-mediated osteoclast formation was also done using a co-culture system as described in Materials and Methods. Student T-test was performed and P<0.05 was considered significant.