Effect on low dose stress on the miRNA levels in Drosophila melanogaster.. Drosophila melanogaster strain:ork

The young adult males from D. melanogaster subjected to mild perturbation in environmental conditions. The adult flies were subjected to a temporary heat stress (HS), where the test subjects were moved to an incubator set at 32 0C for 2 hours. The HS flies were placed at 22 0C under standard conditions for 1 hour. This is the heat primed condition. The heat primed were then transferred to vials with thin strips of Whatman paper soaked with 5% hydrogen peroxide in 30 % sucrose solution at 22 0C under standard conditions for 18 hours. This is the combined stress condition. The miRNA expression was studied after the primed, combined stress and age-matched no stress control conditions. The mRNA expression was also studied for primed and combined stress conditions. The sequencing was performed on a Illumina Nextseq500.