Effect of LRP1 deletion in vascular mural cells and APOE gentotype on gene expression in mouse brains

To investigate the apoE isoform-dependent role of vascular mural cell (VMC)-LRP1, we generated VMC-specific LRP1 knockout mice (smLrp1-/-), followed by breeding these mice with either APOE3-targeted replacement (TR) or APOE4-TR mice We then performed gene expression profiling analysis using data obtained from RNA-seq of cortical samples from 4 different mouse models Comparative gene expression profiling analysis of RNA-seq data for VMC-LRP1 deficiency in the presence of apoE3 or apoE4为探究载脂蛋白E（apolipoprotein E, apoE）亚型依赖的血管壁细胞（vascular mural cell, VMC）-低密度脂蛋白受体相关蛋白1（low-density lipoprotein receptor-related protein 1, LRP1）的作用，我们构建了血管壁细胞特异性LRP1敲除小鼠（smLrp1-/-），随后将该小鼠分别与载脂蛋白E3靶向替换（APOE3-targeted replacement, APOE3-TR）小鼠及载脂蛋白E4靶向替换（APOE4-TR）小鼠交配。随后，我们利用4种不同小鼠模型的皮层样本RNA测序（RNA-seq）数据开展基因表达谱分析，并针对apoE3或apoE4存在条件下VMC-LRP1缺失的RNA测序数据开展对比基因表达谱分析。