Gene expression of human embryonic stem cells during endoderm differentiation. Gene expression of human embryonic stem cells during endoderm differentiation

Using a well-established human definitive endoderm differentiation system, we can analyze the gene expression dynamic of human embryonic stem cells (hESCs) during endoderm differentiation. Samples from hESCs, D2 cells and definitive endoderm cells (DE) were collected for deep sequencing with two replicates for each sample. Differentially expressed genes analysis showed that endodermal genes were induced during endoderm differentiation. Overall design: We performed RNA-seq for human embryonic stem cells (hESCs), endoderm cells induced from hESCs for 2 days (D2) and definitive endoderm cells (DE) in duplicate.