Population genomics reveals local adaptation related to temperature variation in two stream frog species: Implications for vulnerability to climate warming

Identifying populations at highest risk from climate change is a critical component of conservation efforts. However, vulnerability assessments are usually applied at the species level, even though intraspecific variation in exposure, sensitivity, and adaptive capacity play a crucial role in determining vulnerability. Genomic data can inform intraspecific vulnerability by identifying signatures of local adaptation that reflect population-level variation in sensitivity and adaptive capacity. Here, we address the question of local adaptation to temperature and the genetic basis of thermal tolerance in two stream frogs (Ascaphus truei and A. montanus). Building on previous physiological and temperature data, we used whole genome resequencing of tadpoles from four sites spanning temperature gradients in each species to test for signatures of local adaptation. To support these analyses, we developed the first annotated reference genome for A. truei. We then expanded the geographic scope of o..., , , # Population genomics reveals local adaptation related to temperature variation in two stream frog species: Implications for vulnerability to climate warming

[https://doi.org/10.5061/dryad.2280gb638](https://doi.org/10.5061/dryad.2280gb638)

## Description of the data and file structure

**1. Title of dataset:**

Data from: Population genomics reveals local adaptation related to temperature variation in two stream frog species: implications for vulnerability to climate warming

**2. Recommended citation for this dataset:**

Forester BR, Cicchino AS, Shah AA, Mudd AB, Anderson EC, Bredeson JV, Crawford AJ, Dunham JB, Ghalambor CK, Landguth EL, Murray BW, Rokhsar D, Funk WC (2025) Data from: Population genomics reveals local adaptation related to temperature variation in two stream frog species: implications for vulnerability to climate warming, Dryad, Dataset, [https://doi.org/10.5061/dryad.2280gb638](https://doi.org/10.5061/dryad.2280gb638)

**3. Associated paper:**

Forester BR, Cicch...