Impaired angiogenesis in diabetic critical limb ischemia is mediated by a miR-130b-INHBA signaling axis [ischemic gastrocnemius]

We report transcriptomic changes in the EC fraction of the gastrocnemius muscle after intramuscular injection of negative control mimic and miR-130b mimic. we performed intramuscular deliver of negativ control mimic or mir-130b mimic into the ischemic gastrocnemius following femoral artery ligation in db/db mice (4 injections in total). Two weeks post injury, gastrocnemius muscle was harvested and EC fraction was isolated by using CD31 antibody-bound magnetic beads. RNA-Seq analysis was performed using Illumina HiSeq2500 V4 2x150 bp (Genewiz, South Plainfield, NJ).