Differences in H3K4Me3 enrichment between WT and Mist1−/− pancreatic tissue are targeted.

(A) Overlap of called genes identifies subsets of genes that are preferentially enriched for H3K4Me3 in WT or Mist1−/− tissue. (B, C) Schematic representation of ChIP-seq data showing H3K4Me3 enrichment for genes previously published with (B) decreased expression (Cdkn1a, Rab26) or (C) increased expression (Cckar) in Mist1−/− (M1-) pancreatic tissue. (D, C) Schematic representation of ChIP-seq data and validation by ChIP-qPCR showing H3K4Me3 enrichment for genes enriched for H3K4Me3 in (D) WT (Nptx2, Egf) or (E) Mist1−/− tissue (Tm4sf4, Ddc). Arrows indicate peak differences. Bars under the graph depict the extent of the genes. Scale bar = 50 kb (B, C) or 10 kb (D, E). Graphs represent ChIP-qPCR data verifying the differences in H3K4Me3 enrichment for each gene (n = 3). ChIP-qPCR was normalized to input levels. *P