Single cell sequencing data of developing human intestine tissue and human and chimpanzee intestine organoids

To study the species difference in developing intestine between human and chimpanzees, we performed scMultiome profiling on developing human intestine tissues and matched intestinal epithelial only organoids (also known as enteroids), and performed scRNA-seq and scATAC-seq measurements on human and chimpanzee pluripotent stem cell derived intestinal multilineage organoids. We have in vitro organoids and organoids that are further transplanted into mice for further maturation. We also performed scSTARR-seq based on developing human enteroids to quantify the enhancer activity of selected regulatory regions at different epithelial cell types or with different genetic variants.