Investigation of molecular events underlying the miR192-mediated modulation of inflammatory response

To understand the molecular mechanism underlying the miR192-mediated modulation of inflammatory response, we have employed whole genome microarray expression profiling of bone-marrow derived macrophages transfected with miR192. In order to distingish the effect of miR192 on inflammatory response from the effecct of IL-6, RNA was isolated from miR192-transfected and R848-stimulated macrophages in the presence or absence of anti-IL-6 Ab during the culture. The differential gene expressions in mouse bone marrow-derived macrophages transfected with control mimic vs miR192 mimic, tin the presence of control Ab or anti-IL-6 Ab during the in vitro culture. Macrophages in each condition were stimulated with TLR7/8 ligand, R848.