Anatomical-spatial dissection of single-cell retinal transcriptomics paves the road for new horizons in complement immunology [Mm]

Purpose: We present the first comprehensive map of the murine retinal complement expression at a single-cell resolution from ~92,000 retinal cells, spanning 11 cell types. Methods: Retinal mRNA profiles from 10-week-old wild-type (WT) Black 6 mouse eyes were generated by scRNA sequencing. The data was normalized using Seurat. Results: Using an optimized data analysis workflow, we found novel patterns of complement expression in the retina Conclusions: Our landmark atlas provides crucial mechanistic insights in complement immunology Overall design: Retinal cell mRNA data from 10-week old wild type (WT) Black 6 eyes were generated by scRNA sequencing. ~92,000 retinal cells, spanning 11 cell types (Amacrine Cells, Cone BCs, Cone Photoreceptors, Endothelial Cells, Horizontal Cells, Microglia, Muller Glia, Pericytes, Retinal Ganglion Cells, Rod BCs, Rod Photoreceptors).