Structural and functional differences of gut microbiota in Pomacea canaliculata from different geographical locations and habitats

Gut microbiota is related to host fitness, and influenced by geographical locations and habitats. Pomacea canaliculata is a malignant invasive alien snail that threatens agricultural production and ecosystem functions worldwide. Clarifying the general rules of the gut microbial community structure and function of the snails in different geographical locations and habitats is of great significance for understanding their invasion at different spatial scales. This study used high-throughput sequencing technology to compare and analyze the differences in community structure and function of gut microbiota in P. canaliculata from five geographical locations (Liuzhou, Yulin, Nanning, Wuzhou, and Hezhou) and three different habitats (pond, paddy field, and ditch) in Guangxi Province. The results showed that the intestinal microbial alpha diversity of P. canaliculata was higher in Liuzhou, Yulin, lower in Nanning, Wuzhou, and Hezhou, and higher in ponds compared with paddy fields and ditches. T..., Sample collection
In August to September 2022, we collected P. canaliculata in the field from 5 geographical locations (Nanning, NN (107°77′E, 23°09′N); Liuzhou, LZ (109°31′E,  24°37′N); Yulin, YL (109°99′E, 22°32′N); Wuzhou, WZ (110°30′E, 23°54′N); Hezhou, HZ (111°67′E, 24°35′N)) in Guangxi Province, China (Figure 1A). Three sites containing 3 habitats (pond, paddy field, ditch, Figure 1D, E, F) simultaneously were randomly selected in each geographical location, and 5 quadrats (1m2) were set in each habitat (Figure S3). One adult snail was taken from each quadrat for intestinal sample collection, and the distance between the sample quadrat was about 10 meters. We distinguished between male and female when sampling the snails and 23 female snails and 22 male snails were collected from each geographical location. A total of 225 P. canaliculata were collected in this study (5 geographical locations Í 3 sites Í 3 habitats Í 5 replicates). All the testing snails collected from five geograp..., , # **Structural and functional differences of gut microbiota in *Pomacea canaliculata* from different geographical locations and habitats**

## Description of the data and file structure

[https://doi.org/10.5061/dryad.4f4qrfjmk](https://doi.org/10.5061/dryad.4f4qrfjmk)

From August to September 2022, we collected *Pomacea canaliculata* in the field from five geographical locations (Nanning, NN; Liuzhou, LZ; Yulin, YL; Wuzhou, WZ; Hezhou, HZ) in Guangxi Province, China. Three sites containing 3 habitats (pond, paddy field, ditch) simultaneously were randomly selected in each geographical location, and 5 quadrats (1m * 1m) were set in each habitat. One adult snail was taken from each quadrat for intestinal sample collection. A total of 225 snails were collected in this study. The entire intestinal contents of each snail were extracted into a sequence to get the gut microbiota data, which was the dataset of this study.

ASVtable is the Amplicon Sequence Variants (ASVs) of the 225 *Pomacea ...