Analysis of the effect of BCR/CD40/IFN-γ/IL21 on human B cells at gene expression level. Overall design: Total RNA samples were obtained from human memory B cells subjected to 24 hours of in vitro cul

NIAID Data Ecosystem30

Specific primers used for single-cell RT-PCR.

细胞基因表达

PCR引物设计

Specific primers used for single-cell RT-PCR.

Figshare2016-09-28 更新10

Data on direct lysis microplate RT-qPCR assay optimisation and applications

PCR方法优化

细胞基因表达

Fig1_RT.xlsxOptimisations of thermocycler time and reaction mix Fig2_WB.svgCorrelation of chemiluminescent western blot signal to cell lysate loading. Human fibroblasts were lysed and processed by enh

NIAID Data Ecosystem70

Enriched Gene Set Enrichment Analysis categories (FDR-adjusted P-value 10 transcript isoforms within category) encompassing transcript isoforms under-expressed in microglia relative to macrophages.

差异基因表达分析

神经免疫互作

Enriched Gene Set Enrichment Analysis categories (FDR-adjusted P-value 10 transcript isoforms within category) encompassing transcript isoforms under-expressed in microglia relative to mac

NIAID Data Ecosystem30

E-MTAB-9164

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