Expression of target (tissue factor) and housekeeping (beta-actin) genes in purified eosinophils obtained from 9 patients with hypereosinophilia and 9 normal controls.

Gene expression was analysed by real-time polymerase chain reaction and reported as cycle threshold (Ct) and as corrected Ct (2ΔCt = 2Ct tissue factor - Ct beta actin).
* Median value of tissue factor cycle threshold (Ct) was significantly lower in patients with hypereosinophilia than in healthy subjects (p = 0.002). **Median value of tissue factor Ct corrected for beta actin Ct (using the equation 2ΔCt = 2Ct tissue factor - Ct beta actin) was significantly higher in patients with hypereosinophilia than in healthy subjects (p = 0.0001). Both analyses indicate an increased mRNA espression of tissue factor in patients with hypereosinophilia.
Expression of target (tissue factor) and housekeeping (beta-actin) genes in purified eosinophils obtained from 9 patients with hypereosinophilia and 9 normal controls.