Additional file 1 of BIRC5 regulates inflammatory tumor microenvironment-induced aggravation of penile cancer development in vitro and in vivo

Additional file 1: Fig. S1. Silencing BIRC5 inhibited cell viability, migration, invasion of PC cells under IFN-γ treatment (20 ng/mL, 24 h).PC cellswere transfected with shRNA lentiviral vectors. (A and B) Cell viability was studied by CCK-8 assay. (C and D) Wound healing assay and Transwell assay were performed to detect cell migration and invasion. The columns were presented as the mean ± SEM (n ≥ 3). * and**stood forp 0.05 and p 0.01. Fig. S2. Silencing BIRC5 depressed the aggravated tumor growth and survival of PC xenograft mice induced by IFN-γ.Penl1 cells in different groups (IFN-γ; IFN-γ + BIRC5 shRNAb) and untreated Penl1 cells (model) were subcutaneously inoculated at the right axilla (100 µL containing 1 × 106 cells) after mice were anesthetized. (A) Image of tumor growth in living mice. (B, C and D) Tumor volume and tumor weight were measured and survival rate was calculated. The columns were presented as the mean ± SEM. ** stands for p 0.01 compared with model group. ## stands for p 0.01 compared with IFN-γ group. n= 5 in every group.