PyMT 4 stages tumor progression ERRBS (Enhanced Reduced Representation Bisulphite Sequencing )

We sequenced mammary tissues of PyMT mice from 4 stages (hyperplasia at week 6, adenoma/MIN at week 8, early carcinoma at week 10, and late carcinoma at week 12) during tumor progression. We also sequenced mammary tissues of age-matched FVB controls. We sacrificed 3 PyMT mice and 3 FVB control mice at each of the 4 stages, extracted genomic DNA and performed sequencing.